Exercise 8.4: Data collapse¶
Rob Phillips wrote a review paper that I enjoyed entitled “Napoleon is in Equilibrium.”. In the paper, he demonstrated that when you plot data in a certain way, they demonstrate data collapse. The idea here is that if you choose the right thing to plot on the \(x\) and \(y\) axes, data from a variety of sources collapse onto a single universal curve. In this exercise, you will hone your Pandas, NumPy, and Bokeh skills in making plots exhibiting data collapse.
This analysis comes from Rob’s paper, and the data come from Daber, Sochor, and Lewis, J. Mol. Biol., 409, 76–87, 2011. The authors were studying how different mutants of the lac repressor affect gene expression. They hooked the lac promoter up to a fluorescent protein reporter. They made a mutant with no lac repressor to get a measurement of the gene expression level (quantified by the fluorescent signal) in the absence of repressor. Then then looked at how the presence of a repressor served to decrease the expression level of the lac gene. The ratio of the repressed fluorescence to the totally unrepressed fluorescence is the fold change in repression. They can block repression by adding IPTG, which binds the lac repressor, rendering it ineffective at repressing gene expression (so IPTG is called an “inducer,” since it turns on gene expression). So, for a given experiment, the authors measured fold change as a function of IPTG concentration. They measured the fold change for wild type, plus two mutants, Q18M and Q18A.
We will not derive it here (it comes from a generalization of the Monod-Wyman-Changeux model), but the theoretical expression for the fold change as a function of IPTG concentration, \(c\), is
\begin{align} \text{fold change} = \left[1 + \frac{\frac{R}{K}\left(1 + c/K_\mathrm{d}^\mathrm{A}\right)^2}{\left(1 + c/K_\mathrm{d}^\mathrm{A}\right)^2 + K_\mathrm{switch}\left(1 + c/K_\mathrm{d}^\mathrm{I}\right)^2}\right]^{-1}. \end{align}
The parameters are:
Parameter |
Description |
Value |
Units |
|---|---|---|---|
\(K_\mathrm{d}^\mathrm{A}\) |
dissoc. const. for active repressor binding IPTG |
0.017 |
mM\(^{-1}\) |
\(K_\mathrm{d}^\mathrm{I}\) |
dissoc. const. for inactive repressor binding IPTG |
0.002 |
mM\(^{-1}\) |
\(K_\mathrm{switch}\) |
equil. const. for switching active/inactive |
5.8 |
— |
\(K\) |
dissoc. const. for active repressor binding operator |
? |
per cell |
\(R\) |
number of repressors in cell |
? |
— |
The values of \(K_\mathrm{d}^\mathrm{A}\), \(K_\mathrm{d}^\mathrm{I}\), and \(K_\mathrm{switch}\) were measured in the Daber, Sochor, and Lewis paper, and, as I mentioned before, are the same for all mutants. You can see in the expression for the fold change that \(R\) and \(K\) always appear as a ratio, \(R/K\), so we can only determine this ratio, \(R/K\), for each mutant. They are, for the respective mutants:
Mutant |
\(R/K\) |
|---|---|
WT |
141.5 |
Q18A |
16.56 |
Q18M |
1332 |
Now let’s get started with the analysis.
a) Load in the three data sets. They are in the files ~/git/data/wt_lac.csv, ~/git/data/q18m_lac.csv, and ~/git/data/q18a_lac.csv. You should put them in a single DataFrame with an added column for genotype. This can be accomplished, for example, with pd.concat().
b) Make a plot of fold change vs. IPTG concentration for each of the three mutants. Think: should any of the axes have a logarithmic scale?
c) Write a function with the signature fold_change(c, RK, KdA=0.017, KdI=0.002, Kswitch=5.8) to compute the theoretical fold change. It should allow c, the concentration of IPTG, to be passed in as a NumPy array or scalar, and RK, the \(R/K\) ratio, must be a scalar. Remember, with NumPy arrays, you don’t have to write for loops to do operations to each element of the array.
d) You will now plot a smooth curve showing the theoretical fold change for each mutant. >1. Make an array of closely spaced points for the IPTG concentration. Hint: The function np.logspace() will be useful. 2. Compute the theoretical fold change based on the given parameters using the function you wrote in part (c). 3. Plot the smooth curves on the same plot with the data.
e) If we look at the functional form of the fold change and at the parameters we are given, we see that only \(R/K\) varies from mutant to mutant. I told you this a priori, but we didn’t really know it. Daber, Sochol, and Lewis assumed that the binding to IPTG would be unaltered and the binding to DNA would be altered based on the position of the mutation in the lac repressor protein. Now, if this is true, then \(R/K\) should be the only thing that varies. We can check this by seeing if the data collapse onto a single curve. To see how this works, we define the Bohr parameter, \(F(c)\), as
\begin{align} F(c) = -\ln\left(R/K\right) - \ln\left(\frac{\left(1 + c/K_\mathrm{d}^\mathrm{A}\right)^2}{\left(1 + c/K_\mathrm{d}^\mathrm{A}\right)^2 + K_\mathrm{switch}\left(1 + c/K_\mathrm{d}^\mathrm{I}\right)^2}\right). \end{align}
The second term in the Bohr parameter is independent of the identity of the mutant, and the first term depends entirely upon it. Then, the fold change can be written as
\begin{align} \text{fold change} = \frac{1}{1 + \mathrm{e}^{-F(c)}}. \end{align}
So, if we make our \(x\)-axis be the Bohr parameter, all data should fall on the same curve. Hence the term, data collapse. (The Bohr parameter gets its name (as given by Rob Phillips) because it is inspired by the work of Christian Bohr (Niels’s father), who discovered similar families of curves describing binding of oxygen to hemoglobin.)
Now, we will plot the theoretical curve of fold change versus Bohr parameter.
Write a function with call signature
bohr_parameter(c, RK, KdA=0.017, KdI=0.002, Kswitch=5.8)that computes the Bohr parameter.Write a function with call signature
fold_change_bohr(bohr_parameter)that gives the fold change as a function of the Bohr parameter.Generate values of the Bohr parameter ranging from \(-6\) to \(6\) in order to make a smooth plot.
Compute the theoretical fold change as a function of the Bohr parameter and plot it as a gray line.
f) Now, for each experimental curve:
Convert the IPTG concentration to a Bohr parameter using the given parameters.
Plot the experimental fold change versus the Bohr parameter you just calculated. Plot the data as dots on the same plot that you made the universal gray curve, making sure to appropriately annotate your plot.
Do you see data collapse? Does it make sense that only operator binding is changing from mutant to mutant? And importantly, the collapse demonstrates that all of the mutants are behaving according to the Monod-Wyman-Changeux model, and the mutations affect quantitative, not qualitative, changes in the behavior of the repressor.